Does the naked DNA contain one of the following sequences?
a) Sequences of viral origin (see explanation), with the exception of the following sequences:
- a CMV promoter
- an RSV promoter
- an SV40 polyadenylation signal
- an SV40 nuclear targeting sequence
b) Antibiotic-resistance genes, other than those conferring resistance solely to kanamycin or neomycin
Click on the correct answer:
The excepted viral sequences are specified below. This information is also provided in the standard environmental risk assessment for naked DNA.
- The CMV promoter is the Human cytomegalovirus (HCMV, HHV-5) major immediate-early protein gene promoter with a sequence of a maximum length such as established in the Nucleotide Database of the US National Center for Biotechnology Information (NCBI) (Genbank Accession number M60321).
- The RSV promoter is the Rous sarcoma virus (Schmidt-Ruppin) promoter with a sequence of a maximum length such as established in the Nucleotide Database of the NCBI (Genbank Accession number J02025.1).
- The SV40 polyadenylation signal is a sequence of the Simian virus 40(SV40) with a maximum length of around 200 to 300 base pairs.
- The SV40 nuclear targeting sequence is a sequence with an average length of 100 base pairs of the Simian virus 40 (SV40).